Postirradiation white blood cell recovery in rats: Following single and double (repeated) X‑ray exposure
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Abstract
Purpose: This study aims at determining the effect of radiation on peripheral white blood cells (WBCs) and the time for their full recovery in Albino Wister Rats following single and double (repeated) X-ray exposure so that a safe period for repeated irradiation can conveniently be recommended for any radiographic examination.
Materials and Methods: Twenty one healthy albino Wister Rats of aged between 2–4 month and weighing about 140–200 g were used for this study. They were divided into three Groups A, B, and C. Group B and C were irradiated with X-ray from a diagnostic X-ray machine. Group C was immediately irradiated again with the same exposure factors at about 3–4 min interval. Blood samples from both control and experimental groups (after irradiation) were collected through a period of 30 min to 7 days and subjected to standard
hematologic examination to determine the WBC count and differential leukocytes count.
Results: A sharp fall in WBC was observed in the single-exposed group 30 min following irradiation. This is however more pronounced in the double-exposed group. This fall persisted till 48 h after irradiation. The WBC count returned to normal on the 3rd and 4th day following irradiation for both experimental groups. The maximum repair and recovery recorded were 98.6% for single exposure and 95.9% for double exposure of the normal count.
Conclusion: This study has presented that the recovery of an irradiated peripheral WBC is not 100% and that the effect is more remarkable in cells with double (repeated) irradiation. A safe period of at least 3–7 days should be allowed for the cells to recover from previous irradiation before an examination is repeated. This will serve as a guide to radiographers/radiologist in the management of patients who need follow-up examinations.
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References
1. RSSC. Biological Effects of Ionizing Radiation; 2011. p. 1‑5.
2. ColemanCN, BlakelyWF, Fike JR, MacVittieTJ, MettingNF, MitchellJB, et al. Molecular and cellular biology of moderate‑dose (1‑10 Gy) radiation and potential mechanisms of radiation protection: Report of a workshop at Bethesda, Maryland, December 17‑18, 2001. Radiat Res 2003; 159:812‑34.
3. Citrin D, Cotrim AP, Hyodo F, Baum BJ, Krishna MC, Mitchell JB, et al. Radioprotectors and mitigators of radiation‑induced normal tissue
injury. Oncologist 2010;15:360‑71.
4. Hall E, Giaccia A. Radiobiology for Radiologist. 6th ed. Philadelphia: Lippincott Williams & Wilkins; 2006.
5. Williams JP, McBride WH. After the bomb drops: A new look at radiation‑induced multiple organ dysfunction syndrome (MODS). Int J Radiat Biol 2011;87:851‑68.
6. Fajardo LF, Berthrong M, Anderson RE. Radiation Pathology. New York: Oxford University Press; 2001.
7. Yamonenko SP. Radiobiology of Human and Animal English Edition. Moscow: Mir Publishers; 1988. p. 126‑40.
8. Goldin EM, Neff RD. Lymphocyte depletion in peripheral blood of acutely gamma‑irradiated rats. Int J Radiat Biol Relat Stud Phys Chem
Med 1975;27:337‑42.
9. Brar RS, Sandhu HS, Singh A. Veterinary clinical diagnosis by Laboratory Methods. Ludhiana, India: Kalyani publishe; 2000. p. 334.
10. Ikamaise VC, Chiaganam NO, Dike EU, Ndem BN. Post irradiation cell recovery in rats. Niger J Med Imaging Radiat Ther 2007;1:18‑23.
11. Simović M. Influence of cystaphoses on the flow and outcome of associated radiological injury. Beograd: Vojnomedicinska Akademija,
Specijalistički rad; 1983.
12. Miura G, Awaya H, Matsumoto T, Tanaka N, Matsunaga N. Dose granulocite colonystimulating factor exacerbate radiation‑induced acute lung injury in rats. Rad Med 2000;18:227‑32.
13. Milošević H, Saračević L, Hadžijunuzović D. Radiation changes in peripheral blood of domestic animals. Veterinaria, (Sarajevo) 2004; 53, 2-4:179-92.
14. Boggs D. The kinetics of neutrophils leukocytes in health and disease. Semin Hematol 1967;4:359‑86.
15. Bainton DF, UllyotJL, Farquhar MG. The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. J Exp Med
1971;134:907‑34.
16. Grindem CB. Bone marrow biopsy and evaluation. Vet Clin North Am Small Anim Pract 1989;19:669‑96.
17. Gasper WP. The hemopoietic system. In: Feldman FB, Zinkle GJ, Nemi CJ, editors. Schalm’s Veterinary Hematology. 5th ed. Philadelphia:
Lippincott Williams & Wilkins; 2000. p. 63‑73.
18. Baranov AE, Selidovkin GD, Butturini A, Gale RP. Hematopoietic recovery after 10‑gy acute total body radiation. Blood 1994;83:596‑9.
19. Henry F. Fundamental of Radiation Protection. New York: Wiley Interscience; 1969. p. 111‑7.
20. Bell G, Emslie D, Paterson Colin R. Textbook of Physiology. 10th ed. London, New York: Churchill Livingstone; 1980. p. 104‑18.
21. Frankel R. Radiation Protection for Radiation Technologist. 2nd ed. New York: McGraw Hill Book Company; 1996. p. 18‑30.
22. Rozgaj R, KasubaV, Sentija K, Prlic I. Radiation‑induced chromosomal aberrations and hematological alterations in hospital workers. Occup
Med 1999;49:353‑60.
23. Thrall KD, Lovaglio J, Murphy MK, Cataneo RN, Chaturvedi A, Mundada M, et al. A dose‑dependent hematological evaluation of whole‑body gamma‑irradiation in the göttingen minipig. Health Phys 2013;105:245‑52.
24. Maks CJ, Wan XS, Ware JH, Romero-Weaver AL, Sanzari J K, Wilson J M, et al. Analysis of white blood cell counts in mice after gamma‑ or proton‑radiation exposure. Radiat Res 2011;176:170‑6.
25. Gridley DS, Pecaut MJ, Dutta‑Roy R, Nelson GA. Dose and dose rate effects of whole‑body proton irradiation on leukocyte populations and lymphoid organs: Part I. Immunol Lett 2002;80:55‑66.
26. Pecaut MJ, Gridley DS, Smith AL, Nelson GA. Dose and dose rate effects of whole‑body proton‑irradiation on lymphocyte blastogenesis and hematological variables: Part II. Immunol Lett 2002;80:67‑73.
27. El‑Shanshoury H, El‑Shanshoury G, Abaza A. Evaluation of low dose ionizing radiation effect on some blood components in animal model.
J Radiat Res Appl Sci 2016;9:282‑93.